AREGU February 47/2

Thrasher, Terry N., Hong-Gen Chen, and Lanny C. Keil. Arterial baroreceptors control plasma vasopressin responses to graded hypotension in conscious dogs. Am. J. Physiol. Regulatory Integrative Comp. Physiol. 278: R469– R475, 2000.—We studied the role of cardiac and arterial baroreceptors in the reflex control of arginine vasopressin (AVP) and renin secretion during graded hypotension in conscious dogs. The dogs were prepared with Silastic cuffs on the thoracic inferior vena cava and catheters in the pericardial space. Each experiment consisted of a control period followed by four periods of inferior vena caval constriction, during which mean arterial pressure (MAP) was reduced in increments of ,10 mmHg. The hormonal responses were measured in five dogs under four treatment conditions: 1) intact, 2) acute cardiac denervation (CD) by intrapericardial infusion of procaine, 3) after sinoaortic denervation (SAD), and 4) during combined SAD1CD. The individual slopes relating MAP to plasma AVP and plasma renin activity (PRA) were used to compare the treatment effects using a 2 3 2 factorial analysis. There was a significant (P , 0.01) effect of SAD on the slope relating plasma AVP to MAP but no effect of CD and no SAD 3 CD interaction. In contrast, the slope relating PRA and MAP was increased (P , 0.05) by SAD but was not affected by CD. These results support the hypothesis that stimulation of AVP secretion in response to graded hypotension is primarily driven by unloading arterial baroreceptors in the dog.